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Final ruleTook effect June 10, 2026

Procedures for Transportation Workplace Drug and Alcohol Testing Programs

Built by the Tribune's code from the Federal Register. No AI wrote this page. Published in the Federal Register May 11, 2026. Document 2026-09290. Updated Nov. 11, 2026.

What it does

The U.S. Department of Transportation revises its drug and alcohol testing procedures to require a directly observed urine collection in situations where oral fluid tests are currently required but cannot be conducted because oral fluid testing is not yet available. The rule also updates terminology in these procedures consistent with Executive Order (E.O.) 14168, Defending Women from Gender Ideology Extremism and Restoring Biological Truth to the Federal Government.

The Transportation Department's summary, as published in the Federal Register.

What changed in the regulation

1 part of Title 49 of the Code of Federal Regulations:

49 CFR 40

Wording on eCFR: the day before it took effect and from the day it took effect (first part listed).

Compared with

The Transportation Department has published 10 final rules in 2026 so far, compared with 9 in all of 2025.

Counted by the Tribune from Federal Register records since Jan. 1, 2025.

Before this

1 earlier Federal Register document under regulation identifier 2105-AF28 since Jan. 1, 2025.

What happens next and when

This rule is effective on June 10, 2026.

The agency's dates, word for word.

Document number2026-09290
ActionFinal rule.
AgenciesTransportation Department
PublishedMay 11, 2026
Effective dateJune 10, 2026
Regulation identifier (RIN)2105-AF28
DocketDOT-OST-2021-0093

Source: the Federal Register (public domain). The document in the Federal Register